ANALYSIS OF FACTORS RESPONSIBLE FOR THE REGENERATION TO INTACT-CELLS FROM SPHAEROPLASTS OF SACCHAROMYCES-CEREVISIAE

Authors
KUMARI JA PANDA T
Citation
Ja. Kumari et T. Panda, ANALYSIS OF FACTORS RESPONSIBLE FOR THE REGENERATION TO INTACT-CELLS FROM SPHAEROPLASTS OF SACCHAROMYCES-CEREVISIAE, Bioprocess engineering, 10(1), 1994, pp. 15-20
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Bioprocess engineering → ACNP
ISSN journal
0178515X
Volume
10
Issue
1
Year of publication
1994
Pages
15 - 20
Database
ISI
SICI code
0178-515X(1994)10:1<15:AOFRFT>2.0.ZU;2-U
Abstract
The regeneration of Saccharomyces cerevisiae, NCIM 3288, cells from it s sphaeroplasts were found to be influenced by a number of factors. Th e most suitable conditions of regeneration were also dependent on grow th medium, that is, using malt-extract-glucose-yeast extract-peptone ( MGYP) medium: mannitol 0.7 M, pH 6.5, 30 degrees C and using yeast ext ract-peptone-glucose (YPG) medium: sucrose 0.7 M, pH 5.0 and 30 degree s C. The maximum regeneration frequency was observed in YPG medium.