NONCANONICAL OCT-SEQUENCES ARE TARGETS FOR MOUSE OCT-2B TRANSCRIPTIONFACTOR

We have suggested a random modification method for determining prefera ble binding sites of a DNA-binding protein and applied this method to the Oct-2B transcription factor. Our results indicate that the Oct-2B protein interacts with canonical oct sequence ATGC/TAAAT and degenerat ed sequences which contain TAAT motif in the binding site. We have det ermined nucleotides in the binding sites, involved in the DNA-protein interaction, and the equilibrium dissociation constants K-d for these sequences. These data show that a much greater number of potential tar gets for Oct proteins exist on DNA and changed our view on the gene ex pression regulation by this protein factor.