FLUORESCENCE STUDIES ON INTERACTION BETWEEN PHOSPHO-LHC-II AND SUBCHLOROPLAST PHOTOSYSTEM-1 PREPARATIONS

Chloroplast proteins were phosphorylated under two test conditions: 'w hite light' irradiance alone and 'white light' irradiance with the add ition of glucose and glucose oxidase, used to produce an anaerobic med ium. The interaction of phospho-LHC II with Photosystem I (PS 1) was s tudied for two types of PS I preparation. Changes in the chlorophyll a /b ratio and the ratio of 650 and 680 nm band intensities (E650/E680) in fluorescence excitation spectra were used in calculating the phosph o-LHC II portion which became associated with PS 1. It is shown that t he associated portion of phospho-LHC II varies for each of the PS 1 pr eparations and phosphorylation procedures. Possible conclusions as reg ards the transfer of various sets of LHC II subpopulations under diffe rent phosphorylation procedures and the differences of interaction wit h PS 1 are discussed.