H. Hagiwara et al., COMPETITION BETWEEN MOTOR MOLECULES (KINESIN AND CYTOPLASMIC DYNEIN) AND FIBROUS MICROTUBULE-ASSOCIATED PROTEINS IN BINDING TO MICROTUBULES, The Journal of biological chemistry, 269(5), 1994, pp. 3581-3589
In neuronal cells, microtubule-associated proteins (MAPs) can be class
ified into two distinct groups. One consists of force-producing MAPs,
the main components of which are kinesin and cytoplasmic dynein. The o
ther is composed of fibrous MAPs, which include tau and MAP2. Many stu
dies have been performed on the respective groups to understand their
structures and functions. However, the problem of how the groups inter
act with each other on microtubules is still unresolved. To elucidate
the interaction between kinesin or cytoplasmic dynein and tau or MAP2,
we performed three experiments: competition, motility assay, and cose
dimentation. To distinguish whether the binding competition is caused
by steric hindrance of the projection domains of MAPs or by the compet
ition of the binding sites on microtubules, we used microtubule bindin
g domains of tau and MAP2 as well as native proteins. Our results reve
aled that kinesin or cytoplasmic dynein and tau or MAP2 compete for al
most the same binding domains located on the carboxyl-terminal side of
alpha- and the amino-terminal side of beta-tubulin from the site of s
ubtilisin cleavage. Furthermore, the projection of tau, and probably o
f MAP2, might inhibit the binding of kinesin or cytoplasmic dynein to
microtubules by steric hindrance. These findings will provide a useful
step toward understanding the regulation system of intracellular orga
nelle transport.