MULTICOLOR FLUORESCENCE IN-SITU HYBRIDIZATION TO ORDER SMALL, SINGLE-COPY PROBES ON METAPHASE CHROMOSOMES

In constructing complete human chromosome maps, the relative order of markers and their precise chromosomal location will be combined. Multi colour in situ hybridisation, in which two probes are simultaneously h ybridised to chromosomes and subsequently distinguished, potentially w ill provide both types of information. Using this technique, we have p roduced an ordered map of eight human chromosome 3 DNA markers, using small, single-copy probes that can detect target sequences ranging in size from 4 kb to as little as 500 bp.