Investigations pertaining to psychophysiological stress responses typi
cally employ a preexperimental 'baseline' period. The appropriateness
of this methodology was examined in two studies using heart rate (HR)
measures. In study 1, HR decreased significantly from the beginning to
the end of a 15-min adaptation period, suggesting that the optimal le
ngth for a prestress adaptation period must be at least 15 min for HR
when a stressor is anticipated. Study 2 compared the customary prestre
ss 'baseline' measure to four other possible candidates: two recovery
periods between stressor presentations, a postexperimental time period
, and a recording taken on a different day when no stressors were pres
ented. The prestress measure was significantly higher than recovery an
d postexperimental measures. The postexperimental measure was retained
as the best option for the computation of a baseline score. These stu
dies highlight the factors pertinent to the selection of a suitable ti
me frame for a baseline measure.