DNA isolated from gonadal tissue of five intersex pigs was analyzed fo
r the presence of the Y chromosomal markers ZFY and DYZ1. Four cases s
howed no detectable male-specific DNA-sequences. The fifth case exhibi
ted a weak male-specific band at Southern blot analysis with the human
ZFY probe and showed a male-specific PCR-product with DYZ1 primers, i
ndicating an XX/XY gonadal chimera.