K. Harding et Ee. Benson, A STUDY OF GROWTH, FLOWERING, AND TUBERIZATION IN PLANTS DERIVED FROMCRYOPRESERVED POTATO SHOOT-TIPS - IMPLICATIONS FOR IN-VITRO GERMPLASMCOLLECTIONS, Cryo-letters, 15(1), 1994, pp. 59-66
The dynamics of the regeneration process in plants derived from cryopr
eserved in vitro potato shoot-tips have been examined. The combined af
fects of cryopreservation with different post-thaw recovery media (def
ined by plant growth regulator composition) on plant growth, maturatio
n, flowering, and tuberisation were assessed. Cryopreserved shoot-tips
recovered in a medium containing zeatin, gibberellic acid and indole
acetic acid showed relatively rapid, synchronous rates of plant regene
ration and maturation, whereas shoot-tips regenerated on hormone-free
medium, or media containing auxins and/or gibberellic acid developed a
synchronously. The ability of plants derived from cryopreserved shoot-
tips to produce tubers was not affected by the cryopreservation proces
s, unlike the formation of flowers, which was impaired compared to con
trol, tuber-derived plants. In the context of a working genebank, the
rate of and ability to synchronize growth of early post-thaw plantlets
and their development to mature plants may be important consideration
s in choosing freezing and recovery strategies for the conservation of
potato genetic resources.