A HUMAN MONOCLONAL-ANTIBODY TO HIGH-FREQUENCY RED-CELL ANTIGEN JR(A)

Authors
MIYAZAKI T KWON KW YAMAMOTO K TONE Y IHARA H KATO T IKEDA H SEKIGUCHI S
Citation
T. Miyazaki et al., A HUMAN MONOCLONAL-ANTIBODY TO HIGH-FREQUENCY RED-CELL ANTIGEN JR(A), Vox sanguinis, 66(1), 1994, pp. 51-54
Citations number
21
Categorie Soggetti
Hematology
Journal title
Vox sanguinis → ACNP
ISSN journal
00429007
Volume
66
Issue
1
Year of publication
1994
Pages
51 - 54
Database
ISI
SICI code
0042-9007(1994)66:1<51:AHMTHR>2.0.ZU;2-Z
Abstract
A human-mouse heterohybridoma (HMR0921) secreting human monoclonal IgG 3, lambda antibody was produced from peripheral blood lymphocytes of a healthy blood donor with serum antibody to Jr(a), by EBV transformati on and hybridization with mouse myeloma cell line P3X63Ag8.653. The re activity of HMR0921 antibody was assessed by antiglobulin test with a panel of red cells including 14 different rare blood types. Only Jr(a- ) red cells were negative. The strict specificity of this antibody to Jr(a) antigen was further confirmed by absorption test with fluorescen ce flow cytometry. On screening of 28,744 blood donor samples by HMR09 21 antibody, we detected 19 agglutination-negative samples, which were confirmed as Jr(a-) by conventional anti-Jr(a) antisera. Therefore, o ur HMR0921 antibody is extremely useful for detecting rare Jr(a-) bloo d.