CHEMICAL MODIFICATION OF ASPERGILLUS 1,2-ALPHA-D-MANNOSIDASE BY WATER-SOLUBLE CARBODIIMIDE

Authors
YOSHIDA T SHIMIZU S ICHISHIMA E
Citation
T. Yoshida et al., CHEMICAL MODIFICATION OF ASPERGILLUS 1,2-ALPHA-D-MANNOSIDASE BY WATER-SOLUBLE CARBODIIMIDE, Phytochemistry, 35(1), 1994, pp. 267-268
Citations number
17
Categorie Soggetti
Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
35
Issue
1
Year of publication
1994
Pages
267 - 268
Database
ISI
SICI code
0031-9422(1994)35:1<267:CMOA1B>2.0.ZU;2-M
Abstract
1,2-alpha-D-Mannosidase from Aspergillus saitoi was inactivated by che mical modification using water-soluble carbodiimide -ethyl-3-(3-dimeth ylaminopropyl)cardodiimide)(EDC) and glycine ethyl ester (GEE). The in activation rate increased in proportion to the concentration of EDC. K inetic analysis suggested that inactivation of the enzyme occurred by modification of one essential carboxyl group.