EXPRESSION OF INTRON-ENCODED MATURASE-LIKE POLYPEPTIDES IN POTATO CHLOROPLASTS

The trnK gene has been identified on a cloned plastid DNA fragment of potato (Solanum tuberosum cv Desiree). This gene codes for a tRNA-Lys and is interrupted by a 2.5-kb intron belonging to the group II organe lIar introns. In addition, this intervening sequence contains a long o pen reading frame potentially coding for a 509 amino-acid polypeptide (ORF509) related to mitochondrial intron-encoded maturases from fungi. The translational capacity of the trnK intron was first demonstrated in vitro in a prokaryotic DNA-directed expression system. In order to examine the expression of the intron in the potato plant, a synthetic peptide corresponding to the last nine amino acids of the predicted OR F509 product was used to raise antibodies. Western-blot experiments on chloroplast protein extracts, using a sensitive chemiluminescent dete ction system, identified polypeptides similar to in-vitro products. Th ese results suggest that the trnK intron is expressed at the protein l evel in the plant. This is the first report of the in-vivo expression of an intron-encoded polypeptide in higher plant plastids.