THE LEU-ENKEPHALIN-ENCODING SEQUENCE DNA-BINDING FACTOR (LEF) IS THE TRANSCRIPTION FACTOR YY1

Citation
G. Bakalkin et al., THE LEU-ENKEPHALIN-ENCODING SEQUENCE DNA-BINDING FACTOR (LEF) IS THE TRANSCRIPTION FACTOR YY1, Biochemical and biophysical research communications, 231(1), 1997, pp. 135-139
Citations number
19
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
231
Issue
1
Year of publication
1997
Pages
135 - 139
Database
ISI
SICI code
0006-291X(1997)231:1<135:TLSDF(>2.0.ZU;2-K
Abstract
The Leu-enkephalin-encoding sequence DNA-binding factor (LEF) with hig h affinity for the Leu-enkephalin-encoding sequences in the prodynorph in and proenkephalin genes has earlier been identified. This factor is composed of three subunits of about 60, 70 (the major DNA-binding sub unit), and 95 KDa, respectively. Estimated molecular mass, sequence sp ecificity of DNA-binding, and supershift/inhibition with specific anti bodies in a band shift assay showed that the DNA-binding subunit of LE F is identical to the multifunctional transcription factor YY1. Howeve r, an antibody against the C-terminus of YY1 distinguished the YY1 com plexes with a Leu-enkephalin-encoding sequence and canonical YY1 bindi ng site oligonucleotides, suggesting different protein conformations i n complexes with these two DNA fragments. (C) 1997 Academic Press.