G. Bakalkin et al., THE LEU-ENKEPHALIN-ENCODING SEQUENCE DNA-BINDING FACTOR (LEF) IS THE TRANSCRIPTION FACTOR YY1, Biochemical and biophysical research communications, 231(1), 1997, pp. 135-139
The Leu-enkephalin-encoding sequence DNA-binding factor (LEF) with hig
h affinity for the Leu-enkephalin-encoding sequences in the prodynorph
in and proenkephalin genes has earlier been identified. This factor is
composed of three subunits of about 60, 70 (the major DNA-binding sub
unit), and 95 KDa, respectively. Estimated molecular mass, sequence sp
ecificity of DNA-binding, and supershift/inhibition with specific anti
bodies in a band shift assay showed that the DNA-binding subunit of LE
F is identical to the multifunctional transcription factor YY1. Howeve
r, an antibody against the C-terminus of YY1 distinguished the YY1 com
plexes with a Leu-enkephalin-encoding sequence and canonical YY1 bindi
ng site oligonucleotides, suggesting different protein conformations i
n complexes with these two DNA fragments. (C) 1997 Academic Press.