Characteristic changes in proton nuclear magnetic resonance (NMR H-1-N
MR) signals occur when winegrape buds, responding to their environment
, proceed through endo- (approximately rest) and ecodormancy (approxim
ately quiescence). These NMR signals were processed using a recently d
eveloped method Gardea et al., 1992, Spectroscopy, 8 (1): 27-35), that
synchronizes second derivative minima allowing fine resolution of sig
nal complexes, and uses careful orientation of the bud in the magnetic
field to maximize signal resolution arising from McCain anisotropy (M
cCain et al., 1984. Proc. Natl. Acad. Sci. USA, 81: 748-752). Changes
in H-1-NMR signals associated with changes in dormancy, spectroscopy m
easurement temperatures (from 25 to -30-degrees-C) and centrifugal rem
oval of water are described. An H-1-NMR signal, tentatively associated
with the presence of intact large vacuoles, is discussed.