PROTON NMR SIGNALS ASSOCIATED WITH ECODORMANCY AND ENDODORMANCY IN WINEGRAPE BUDS

Citation
Aa. Gardea et al., PROTON NMR SIGNALS ASSOCIATED WITH ECODORMANCY AND ENDODORMANCY IN WINEGRAPE BUDS, Scientia horticulturae, 56(4), 1994, pp. 339-358
Citations number
31
Categorie Soggetti
Horticulture
Journal title
ISSN journal
03044238
Volume
56
Issue
4
Year of publication
1994
Pages
339 - 358
Database
ISI
SICI code
0304-4238(1994)56:4<339:PNSAWE>2.0.ZU;2-P
Abstract
Characteristic changes in proton nuclear magnetic resonance (NMR H-1-N MR) signals occur when winegrape buds, responding to their environment , proceed through endo- (approximately rest) and ecodormancy (approxim ately quiescence). These NMR signals were processed using a recently d eveloped method Gardea et al., 1992, Spectroscopy, 8 (1): 27-35), that synchronizes second derivative minima allowing fine resolution of sig nal complexes, and uses careful orientation of the bud in the magnetic field to maximize signal resolution arising from McCain anisotropy (M cCain et al., 1984. Proc. Natl. Acad. Sci. USA, 81: 748-752). Changes in H-1-NMR signals associated with changes in dormancy, spectroscopy m easurement temperatures (from 25 to -30-degrees-C) and centrifugal rem oval of water are described. An H-1-NMR signal, tentatively associated with the presence of intact large vacuoles, is discussed.