Albumin-heparin microspheres were prepared by a two-step process which
involved the preparation of a soluble albumin-heparin conjugate, foll
owed by formation of microspheres from this conjugate or by a double c
ross-linking technique involving both coupling of soluble albumin and
heparin and microsphere stabilization in one step. The first technique
was superior since it allowed better control over the composition and
the homogeneity of the microspheres. Microspheres could be prepared w
ith a diameter of 5-35 mu m. The size could be controlled by adjusting
the emulsification conditions. The degree of swelling of the microsph
eres was sensitive to external stimuli, and increased with increasing
pH and decreasing ionic strength of the medium.