V. Trinkausrandall et al., EFFECT OF PRETREATING POROUS WEBS ON STROMAL FIBROPLASIA IN-VIVO, Journal of biomedical materials research, 28(2), 1994, pp. 195-202
We have previously demonstrated that keratocytes penetrate and deposit
collagen after a porous web is inserted into interlamellar corneal po
ckets. In these studies our goal was to determine whether pretreatment
of the porous discs would enhance wound healing. We have evaluated fo
ur methods of pretreating the porous disc prior to its placement in th
e stroma. The pretreated discs were followed in vivo for a period of 4
2 days. The criteria we used to determine whether pretreatment affecte
d wound healing were: collagen deposition, extent of fibroplasia, the
synthetic rate of keratocytes within the disc, and lack of edema. Our
results indicate that preseeding with stromal keratocytes enhanced the
overall synthetic rate and specifically enhanced the amount of collag
e deposited within the web. (C) 1994 John Wiley and Sons, Inc.