SUBSTRATE SPECIFICITIES OF EXTRACELLULAR PROTEASES PRODUCED BY MARINEPUTREFACTIVE BACTERIA, SHEWANELLA-PUTREFACIENS AND ALTEROMONAS-HALOPLANKTIS

Authors
ODAGAMI T MORITA J TAKAMA K SUZUKI S
Citation
T. Odagami et al., SUBSTRATE SPECIFICITIES OF EXTRACELLULAR PROTEASES PRODUCED BY MARINEPUTREFACTIVE BACTERIA, SHEWANELLA-PUTREFACIENS AND ALTEROMONAS-HALOPLANKTIS, Letters in applied microbiology, 18(1), 1994, pp. 50-52
Citations number
13
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
18
Issue
1
Year of publication
1994
Pages
50 - 52
Database
ISI
SICI code
0266-8254(1994)18:1<50:SSOEPP>2.0.ZU;2-C
Abstract
Substrate specificities of proteases produced by two putrefactive mari ne bacteria, Shewanella putrefaciens and Alteromonas haloplanktis, wer e surveyed by using peptidyl-7-amino-4-methylcoumarin (MCA-substrates) . Shewanella putrefaciens produced trypsin-like enzyme(s) showing broa d spectrum specificity and chymotrypsin-like enzyme specifically hydro lysing Glt-Gly-Gly-Phe-MCA. Alteromonas haloplanktis produced high act ivity of aminopeptidase and trypsin-like enzyme(s) preferring Z-Phe-Ar g-MCA, Bz-Arg-MCA and Boc-Leu-Ser-Thr-Arg-MCA. The two organisms would be able to utilize different proteins for their growth.