DESIGN OF AN AQUEOUS 2-PHASE SYSTEM FOR THE PURIFICATION OF ICP FROM BACILLUS-THURINGIENSIS

Authors
GUERECA L BRAVO A QUINTERO R
Citation
L. Guereca et al., DESIGN OF AN AQUEOUS 2-PHASE SYSTEM FOR THE PURIFICATION OF ICP FROM BACILLUS-THURINGIENSIS, Process biochemistry, 29(3), 1994, pp. 181-185
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
29
Issue
3
Year of publication
1994
Pages
181 - 185
Database
ISI
SICI code
1359-5113(1994)29:3<181:DOAA2S>2.0.ZU;2-O
Abstract
A method is described for the purification of Bacillus thuringiensis p rotein crystals based on an aqueous two-phase system distribution, com posed of potassium phosphate-polyethylene glycol-water. This method is simple, one step, fast, inexpensive and efficient compared with other techniques. It has been used successfully to purify and characterize the protein crystals from several strains of Bacillus thuringiensis.