T. Henle et al., A SIMPLE METHOD FOR THE PREPARATION OF FUROSINE AND PYRIDOSINE REFERENCE MATERIAL, Zeitschrift fur Lebensmittel-Untersuchung und -Forschung, 198(1), 1994, pp. 66-67
After heating N(alpha)-acetyllysine and glucose for 4 h at 90-degrees-
C in the dry state and subsequent acid hydrolysis with 7.8 N HCl, prep
arative fractionation of the dihydrochlorides of furosine and pyridosi
ne was achieved by cation-exchange chromatography. The lysine derivati
ves could be prepared with high yield and sufficient purity for the us
e as reference material.