DEVELOPMENT OF AN ELISA FOR MEASURING THE ACTIVITY OF TETANUS TOXOID IN VACCINES AND COMPARISON WITH THE TOXIN NEUTRALIZATION TEST IN MICE

Authors
MANGHI MA PASETTI MF BRERO ML DELUCHI S DIPAOLA G MATHET V ERIKSSON PV
Citation
Ma. Manghi et al., DEVELOPMENT OF AN ELISA FOR MEASURING THE ACTIVITY OF TETANUS TOXOID IN VACCINES AND COMPARISON WITH THE TOXIN NEUTRALIZATION TEST IN MICE, Journal of immunological methods, 168(1), 1994, pp. 17-24
Citations number
16
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
168
Issue
1
Year of publication
1994
Pages
17 - 24
Database
ISI
SICI code
0022-1759(1994)168:1<17:DOAEFM>2.0.ZU;2-2
Abstract
An enzyme-linked immunosorbent assay (ELISA) has been developed to mea sure anti-tetanus toroid antibody levels in immunized guinea-pig sera as a useful alternative to the currently used toxin neutralization tes t (TNT) in determining the activity of the tetanus toroid in vaccines. The ELISA was found to measure antibody levels as low as 5.8 x 10(-5) IU/ml. Furthermore, a comparison of the results from ELISA and TNT in volving 132 different commercial vaccines showed a very good correlati on (r = 0.94, p < 0.001) between antibody levels measured by both meth ods. The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in mice for assessing the activ ity of tetanus toxoids in vaccines.