AN ULTRASENSITIVE CHEMILUMINOENZYME IMMUNOASSAY FOR THE QUANTIFICATION OF HUMAN TISSUE KININOGENS - APPLICATION TO SYNOVIAL-MEMBRANE AND CARTILAGE

Authors
LEGRIS F MARTELPELLETIER J PELLETIER JP COLMAN R ADAM A
Citation
F. Legris et al., AN ULTRASENSITIVE CHEMILUMINOENZYME IMMUNOASSAY FOR THE QUANTIFICATION OF HUMAN TISSUE KININOGENS - APPLICATION TO SYNOVIAL-MEMBRANE AND CARTILAGE, Journal of immunological methods, 168(1), 1994, pp. 111-121
Citations number
57
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
168
Issue
1
Year of publication
1994
Pages
111 - 121
Database
ISI
SICI code
0022-1759(1994)168:1<111:AUCIFT>2.0.ZU;2-V
Abstract
A sandwich enzyme immunoassay using a chemiluminescent detection has b een developed for the quantification of total human (high and low mole cular weight) kininogens in tissue extracts. This assay uses monospeci fic polyclonal IgG labelled with alkaline phosphatase and the commerci ally available dioxetane derivatives as substrates, for the detection of immune complexes. This method exhibits a sensitivity level of 1 fmo l/ml and allows a precise quantification of total kininogens in synovi um and cartilage extracts. When characterized by Western blot, the imm unoreactive material reveals the presence of both high and low molecul ar weight kininogens.