TYPE-I DIABETIC PREGNANCY AND SUBCLINICAL HUMAN PAPILLOMAVIRUS INFECTION

It has been suggested that diabetic pregnancy is an immunosuppressive state, To determine whether the possible immunosuppression in pregnant diabetics might result in an increased risk for human papillomavirus (HPV) infection, we studied exfoliated cells from the uterine cervix, vagina, and posterior commissure of the vulva by means of dot blot hyb ridization with use of a probe cocktail of HPV types 11, 16, and 18 un der low stringency and by means of consensus primer-mediated polymeras e chain reaction (PCR) targeted to the HPV L1 and E1 regions, For this study, samples from 31 pregnant diabetics whose glucose levels had be en reasonably well controlled were analyzed during the first trimester ; samples from 27 of these patients were analyzed again during the thi rd trimester, Fifty-one healthy pregnant women were included as contro ls, Only one of the pregnant diabetics was positive for HPV DNA, The L 1 PCR products of the first and third trimester samples from this pati ent were sequenced, and both were found to represent HPV 61, Three of the pregnant controls were positive for HPV: two were positive for HPV 16, and one was positive for HPV 6, The 95% confidence limits for the prevalence of HPV were calculated to be 0.1%-16.7% for the diabetics and 1.2%-16.2% for the controls, The 95% confidence limits for the dif ference between the groups were -11.6%-6.3%, These results suggest tha t pregnant diabetics do not have an increased risk of developing HPV i nfection, at least when their glucose levels remain well controlled.