AXENIC CULTIVATION OF THE FREE-LIVING SOIL AMEBAS, ACANTHAMOEBA-CASTELLANII AND HARTMANNELLA-VERMIFORMIS IN A CHEMOSTAT

Authors
WEEKERS PHH VOGELS GD
Citation
Phh. Weekers et Gd. Vogels, AXENIC CULTIVATION OF THE FREE-LIVING SOIL AMEBAS, ACANTHAMOEBA-CASTELLANII AND HARTMANNELLA-VERMIFORMIS IN A CHEMOSTAT, Journal of microbiological methods, 19(1), 1994, pp. 13-18
Citations number
15
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
19
Issue
1
Year of publication
1994
Pages
13 - 18
Database
ISI
SICI code
0167-7012(1994)19:1<13:ACOTFS>2.0.ZU;2-K
Abstract
A simple procedure for the axenic cultivation of the free-living soil amoebae Acanthamoeba castellanii and Hartmannella vermiformis in chemo stats was developed. Amoebal cell numbers, optical density and protein concentrations were determined to quantitate amoebal cell growth, and respiration rates were determined to assure optimum growth conditions for the cultures. Dilution rates were gradually increased to obtain s teady-state conditions with minimum generation times and maximum cell numbers of approximately 25 and 16 hours, and 2.5 and 3.2 x 10(6) cell s per mi for A. castellanii and H. vermiformis, respectively.