Three possible causes of intersexuality in Gammarus duebeni, a crustac
ean with environmental sex determination, were investigated. Intersexu
ality appears to be a cost of the flexible sex determining mechanism o
f this species: the occurrence of intersexes is influenced by photoper
iod, which also cues sex determination. Intersexuality is heritable: i
ntersex mothers produce more intersex offspring than do true females.
Parasitism by a feminizing microsporidian is ruled out as a significan
t cause of intersexuality.