PRODUCTION OF A THERMOSTABLE ALKALINE PROTEASE BY A NEW PSEUDOMONAS-SP BY SOLID SUBSTRATE FERMENTATION

Parameters for the production of a thermostable alkaline protease by s olid substrate fermentation from a new Pseudomonas sp. B45 have been o ptmized. The organism could elaborate enzyme production on coarse whea t bran medium maximally at 96h when the initial moisture content was m aintained at 74%. The organism could produce stable enzyme in alkaline environment (pH 8.3 - 8.8). Retention of enzyme activity upto 96% as observed when the wheat bran biomass was dried at 65-degrees-C.