This paper deals with the biological removal of urea nitrogen and ammo
nia nitrogen from the urea fertilizer plant effluent In the present st
udy a packed bed reactor immobilized with the culture of Bacillus spha
ericus was utilized to study the effect of pH and temperature on COD r
emoval, Urea-N and Ammonia-N removal. The optimum temperatures and pH
for urea removal was observed at 30-degrees-C and 10-degrees-C respect
ively while in case of ammonia maximum removal was observed at pH 11 a
nd temperature 30-degrees-C . Maximum COD removal was also observed at
temperature 30-degrees-C and pH 11.