Sd. Haley et al., RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER VARIABILITY BETWEEN AND WITHIN GENE POOLS OF COMMON BEAN, Journal of the American Society for Horticultural Science, 119(1), 1994, pp. 122-125
The objective of this study was to evaluate the degree of RAPD marker
variability between and within commercially productive market classes
representative of the Andean and Middle American gene pools of common
bean (Phaseolus vulgaris L.). Six sets of near-isogenic lines were scr
eened with oligonucleotide primers in the polymerase chain reaction-ba
sed RAPD assay. Simultaneous analyses with at least three sets of line
s enabled us to score RAPD markers between the two major gene pools, r
aces within the same gene pool, and different genotypes of the same ra
ce (within race). A ''three-tiered'' pattern of polymorphism was obser
ved: between gene pools > between races > within races. The overall le
vel of polymorphism between the Andean and Middle American gene pools
was 83.4 %. The overall level of polymorphism between races within the
same gene pool was similar for Andean races (60.4%) and Middle Americ
an races (61.7%). The level of polymorphism between related commercial
navy bean tines was 39.2% and between related commercial snap bean li
nes was 53.6%. The inherent simplicity and efficiency of RAPD analyses
, coupled with the number of polymorphisms detectable between related
commercial genotypes, should facilitate the construction of RAPD-based
genetic linkage maps in the context of populations representative of
most bean breeding programs.