M. Chinami et al., FUNCTIONAL OLIGOMERIZATION OF PURIFIED HUMAN PAPILLOMAVIRUS TYPE-16 AND TYPE-6B E7 PROTEINS EXPRESSED IN ESCHERICHIA-COLI, Journal of General Virology, 75, 1994, pp. 277-281
Purified non-fused soluble human papillomavirus type 16 and 6b E7 prot
eins expressed in Escherichia coli were found to form oligomers. For b
oth proteins, several degrees of oligomerization were demonstrated by
gel filtration, dynamic laser light scattering and scanning electron m
icroscopy. Oligomerization was dependent on the concentration of E7 pr
otein. Oligomerized E7 proteins were able to bind the retinoblastoma g
ene product pRB and stimulated DNA synthesis when introduced into cell
s.