Rf. Garry, SINDBIS VIRUS-INDUCED INHIBITION OF PROTEIN-SYNTHESIS IS PARTIALLY REVERSED BY MEDIUM CONTAINING AN ELEVATED POTASSIUM CONCENTRATION, Journal of General Virology, 75, 1994, pp. 411-415
Infection of cultured vertebrate cells by Sindbis virus, an alphavirus
, results in a reduction in the overall rate of protein synthesis and
in selective termination (shutoff) of host-specified protein synthesis
. The shutoff of host protein synthesis by Sindbis virus has been temp
orally correlated with a decrease in intracellular K+ concentration (a
nd an increase in intracellular Na+ concentration) which occurs as a c
onsequence of virus-mediated inhibition of the plasma membrane-associa
ted Na+/K+ ATPase. Incubation of Sindbis virus-infected cells in mediu
m containing an elevated concentration of K+ resulted in an increase i
n the intracellular concentration of K+, an increase in the overall ra
te of protein synthesis, and in partial reversal of the virus-induced
termination of cell-specified protein synthesis. These results suggest
that the virus-induced decrease in intracellular K+ concentration is
required for efficient shutoff of host protein synthesis by Sindbis vi
rus.