The utility of a simple biotyping scheme to differentiate pathogenic a
nd non-pathogenic strains of Yersinia enterocolitica was determined fo
r 79 patients who were admitted to or attended a reference children's
hospital in western Canada. Biotyping defined predominantly two subset
s of Y enterocolitica. 'Pathogenic' strains were more Likely to have b
een obtained from younger patients (mean age 61.9 months) who experien
ced an acute gastrointestinal illness that was occasionally associated
with bloody diarrhoea or a surgical procedure. Growth of Y enterocoli
tica from selective solid bacteriological growth media were often in t
he moderate to heavy range (82.0%). In contrast, 'non-pathogenic' stra
ins were more often obtained from older patients (mean 116.0 months) w
ho were already recognised to have suffered from a chronic illness and
who were likely to have been admitted to hospital. Moderate to heavy
growth of bacterium in stool specimens were infrequently (17.4%) obtai
ned from the latter patients. The use of a simple biotyping scheme for
the differentiation of Y enterocolitica strains has the potential to
improve patient care.