ISOLATION OF NOVEL NON-HLA GENE FRAGMENTS FROM THE HEMOCHROMATOSIS REGION (6P21.3) BY CDNA HYBRIDIZATION SELECTION

It has previously been shown that cDNA hybridization selection can ide ntify and recover novel genes from large cloned genomic DNA such as co smids or YACs. In an effort to identify candidate genes for hemochroma tosis, this technique was applied to a 320-kb YAC containing the HLA-A gene. A short fragment cDNA library derived from human duodenum was s elected with the YAC DNA. Ten novel gene fragments were isolated, char acterized, and localized on the physical map of the YAC.