ADSORPTION OF AEROSIL ON ERYTHROCYTE SURFACE BY FLOW-CYTOMETRY MEASUREMENTS

Light scattering properties of the erythrocytes in less than 0.01% aer osil suspension were measured. It was shown that light scattering prop erties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are char acterized by more intensive autofluorescence in the channel of green f luorescence than the erythrocytes. We propose that this observation al lows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aer osil particles and the erythrocytes, was confirmed by photometric meas urement of hemoglobin in the supernatant. (C) 1994 Wiley-Liss, Inc.