RESTORING AND IMMUNOHISTOLOGICAL EXAMINATION OF FEULGEN STAINED AVIANEMBRYONIC-TISSUES USING IRON HEMATOXYLIN AND ENDOTHELIA CELL-SPECIFICANTIBODY

The immunohistological method described here permits re-examination of previously Feulgen stained quail-chick chimera tissues for vascular d evelopment using the monoclonal antibody QH1 which specifically recogn izes quail hemangioblastic cells, Weigert's iron hematoxylin has been used to restore faded or bleached Feulgen stained chimeric avian tissu es. Species-specific differences in nuclear morphology are as evident with iron hematoxylin staining as they are with Feulgen staining.