CYTOKINE-GENE EXPRESSION IN MEASLES-INFECTED ADULT HUMAN GLIAL-CELLS

The expression of interleukin (IL)-1 beta, IL-6 and tumor necrosis fac tor (TNF) alpha transcripts in cultured human glial cells was examined using reverse transcription followed by polymerase chain reaction (PC R) amplification and Southern blot quantitation. Microglial cultures d erived from brain biopsy specimens from three different individuals ex pressed transcripts for the three cytokines under basal culture condit ions. This expression was enhanced in response to measles virus (MV) i nfection (IL-1 beta, 2.2-8.8-fold; IL-6, 2.5-8.4-fold; TNF alpha, 2.2- 3.2-fold). Neither IL-1 beta nor TNF alpha transcripts were detectable in undissociated brain tissue from two individuals, suggesting that t he basal expression of these cytokines in culture may have been induce d by tissue dissociation or by the culture conditions. Oligodendrocyte s did not express cytokine transcripts under basal culture conditions, and IL-1 beta and IL-6 but not TNF alpha transcripts could be induced by MV. Similarly, meningeal fibroblasts expressed IL-1 beta and IL-6 but not TNF alpha in response to MV-infection, suggesting that the pro duction of TNF alpha is more cell type-restricted than either IL-1 bet a or IL-6. The results indicate that adult human microglia can partici pate in the inflammatory response to MV infection in the CNS by produc ing cytokines that contribute to inflammation and demyelination. In ad dition, besides their role in myelination, oligodendrocytes can potent ially influence immunoreactivity in the CNS by producing IL-1 beta and IL-6.