BLOOD TYPING OF BIOCHEMICAL POLYMORPHISMS AND APPLICATION TO PATERNITY TEST IN HORSES

A total of 543 blood samples of horses from the Thoroughbred (237) and Chilean F.S. (306) breeds, from different farms belonging to the V-th and X(th) Regions were analyzed for 8 different biochemical loci. The biochemical polymorphisms were detected by Horizontal Polyacrylamide gel electrophoresis (Al, Gc, Es, Xk and Tf), Starch gels (Al, Es, 6-PG D and Cat) and Isolectric focusing technique (Hb). Gene frequencies we re calculated for all the polymorphisms detected and the total probabi lity for Paternity exclution was estimated in 0.750 for Thoroughbreds and 0.805 for Chilean F.S. breeds. Based on these results, the applica tion of biochemical polimorphisms to paternity tests would allow a hig h percentage of security in the exclusion of one or both progenitors.