CD8(HIGH)-CELLS IN PATIENTS WITH RHEUMATOID-ARTHRITIS( (CD57+) T)

Objective. To investigate the development and T cell receptor (TCR) us age of CD8+, CD57+ T cells in rheumatoid arthritis (RA) patients. Meth ods. Three-color flow cytometry using monoclonal antibodies (MAb) to C D8, CD57 and different TCR V-beta gene products. Results. The proporti on of CD8+ T cells expressing CD57 (CD57/CD8) was significantly higher in RA patients compared with age-matched controls. Expanded TCR V-bet a populations were more frequent, and were found in both RA patient-de rived CD8(high)+(CD57+) and CD8+, CD57- populations. TCR V(beta)5+ and TCR V(beta)13+ expansions were present at high frequency (5 of 26 and 7 of 26, respectively). TCR V-beta expansions in CD8(high)+(CD57+) ly mphocytes from RA patients were significantly larger than those in age -matched controls (expansion index 2.38 +/- 0.28, n = 41 and 1.63 +/- 0.09, n = 32, respectively), and were stable over time. Conclusion. RA leads to an increase in the frequency of expanded CD8+ T cell subsets expressing selected TCR, due to expansion of TCR V-beta+ populations in CD8(high)+(CD57+) T cells. Their restricted TCR usage suggests pote ntial specificity for RA antigens and, therefore, a potential role in the pathogenesis of RA.