DIFFERENCES IN THE MACROMOLECULAR-COMPOSITION OF ZONA-PELLUCIDA ISOLATED FROM BOVINE OOCYTES, EMBRYOS AND A23187 PRETREATED OOCYTES

Authors
BERCEGEAY S ALLAIRE F JEAN M LHERMITE A BRUYAS JF RENARD N TAINTURIER D BARRIERE P
Citation
S. Bercegeay et al., DIFFERENCES IN THE MACROMOLECULAR-COMPOSITION OF ZONA-PELLUCIDA ISOLATED FROM BOVINE OOCYTES, EMBRYOS AND A23187 PRETREATED OOCYTES, Reproduction, nutrition, development, 33(6), 1993, pp. 567-576
Citations number
46
Categorie Soggetti
Reproductive Biology","Nutrition & Dietetics","Developmental Biology
Journal title
Reproduction, nutrition, development → ACNP
ISSN journal
09265287
Volume
33
Issue
6
Year of publication
1993
Pages
567 - 576
Database
ISI
SICI code
0926-5287(1993)33:6<567:DITMOZ>2.0.ZU;2-T
Abstract
The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight ((MW) of 80-70 kD, 66-63 kD and 60 kD, respectively a s revealed by I-dimensional SDS-PAGE. After P-dimensional SDS-PAGE, th e zona pellucida electrophoretic pattern indicates a fourth glycoprote in, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic com position.