HEPATITIS-C VIRUS-INFECTION DETECTED BY ANTIBODY TESTS AND THE POLYMERASE CHAIN-REACTION AS A CAUSE OF LIVER DYSFUNCTION IN RENAL-TRANSPLANT RECIPIENTS
Eab. Mccruden et al., HEPATITIS-C VIRUS-INFECTION DETECTED BY ANTIBODY TESTS AND THE POLYMERASE CHAIN-REACTION AS A CAUSE OF LIVER DYSFUNCTION IN RENAL-TRANSPLANT RECIPIENTS, Journal of medical virology, 42(2), 1994, pp. 158-163
Hepatitis C infection (HCV) is more prevalent in patients who have rec
eived kidney transplants than in the general population but the morbid
ity and mortality associated with infection in this group is unclear.
Sera taken from 36 renal transplant recipients with chronic liver dysf
unction and from 42 with normal liver function were tested for HCV inf
ection by second generation ELISA (Abbott Laboratories) and second gen
eration recombinant immunoblot assay (Chiron Corporation) (RIBA-2). Ev
idence of HCV replication was sought by reverse transcription polymera
se chain reaction (RT PCR) using primers from the 5' nontranslated reg
ion (5'NTR). Infection was detected in 20/36 (54%) and in 2/42 (4.8%)
controls (P < 0.01). Twelve liver dysfunction patients were positive b
y all three tests, six were positive by ELISA and RT PCR but had indet
erminate RIBA-2, one was positive by ELISA and RIBA but negative by RT
PCR, and one was positive only by RT PCR. Of two infected control pat
ients, one was positive by all three tests and one who was later found
to have been in the early stage of infection was positive only by RT
PCR. Follow-up of infected patients showed persistence of viraemia in
14/15 (93%). Evidence of infection with different types of HCV was sho
wn by the lack of amplification by RT PCR by primers with mismatching
bases with HCV types 2 and 3. It is concluded that in our renal transp
lant patients, chronic HCV infection is usually associated with liver
dysfunction and persistent infection is common, (C) 1994 Wiley-Liss, I
nc.