CHANGES IN CHEMICAL-COMPONENTS AND ENERGY-CHARGE DURING GROWTH-CYCLE OF CHATTONELLA-ANTIQUA

The changes in cellular content of adenosine triphosphate (ATP) in Cha ttonella antiqua (Hada) Ono (Raphidophyceae), a red tide microorganism , were determined during growth in laboratory culture using ESM-enrich ed seawater. Carbon, nitrogen, and phosphorus in the cells were also a nalyzed. The cellular content of ATP decreased within several hours Of inoculation into fresh medium, then rapidly increased during the earl y exponential Phase of growth to 20-30 pg ATP cell-1. After the mid ex ponential phase ATP decreased and reached a constant level from the la te exponential phase to the stationary phase at 5-10 Pg ATP cell-1. Th e decrease of ATP after inoculation seemed to be due to a lack of extr acellular organic materials in the fresh medium. The addition of both low and high molecular fractions obtainned by dialysis from early stat ionary phase cultures of C. antiqua increased the cellular ATP content in the early exponential phase. The energy charge decreased from 0.63 to about 0.5 during the lag phase and recovered during the exponentia l phase. The lag phase in C. antiqua culture is the period of adaptati on to fresh medium possibly by the production of extracellular organic materials at the expense of cellular ATP.