EFFECTS OF STORAGE-CONDITIONS ON ELECTROP HOREGRAMS OF PROTEINS AND LDH ISOZYMES FROM SKELETAL-MUSCLE AND VITREOUS-HUMOR OF OCELLATE PUFFER

The proteins in skeletal muscle extract and vitreous humor from Takifu gu rubripes were separated by polyacrylamide gel isoelectric focusing (PAG-IEF), and their L-lactate dehydrogenase (LDH) isozymes were separ ated by agarose gel electrophoresis. The electrophoregrams of proteins and LDH isozymes showed specific patterns for each of skeletal muscle and vitreous humor. These patterns were not significantly different a mong 10 specimens of pufferfish. The protein patterns of skeletal musc le were changed by storage at 4-degrees-C, but not at all by storage a t -20-degrees-C for 24 weeks. The protein patterns of vitreous humor w ere markedly changed by storage even at -20-degrees-C. However, the LD H zymogram of vitreous humor was not significantly changed by storage at 4-degrees-C for 1 week or at -20-degrees-C for 24 weeks. Thus the e lectrophoretic analyses of skeletal muscle proteins and of LDH isozyme s from skeletal muscle and vitreous humor of pufferfish are assumed to be useful for the distinction of pufferfish species even after storag e at -20-degrees-C for 24 weeks.