THE GENE FOR THE IRON-SULFUR PROTEIN OF SUCCINATE-DEHYDROGENASE (SDH-IP) MAPS TO HUMAN-CHROMOSOME 1P35-36.1

A partial human cDNA clone for the iron-protein (IF) subunit of succin ate dehydrogenase (EC 1.3.99.1) was used in Southern analyses of restr iction enzyme digests of genomic human and hamster DNA as well as hams ter-human hybrids containing a limited number of human chromosomes. Th e gene for this protein was mapped to human chromosome 1. Digestion of genomic DNA with several restriction enzymes yielded two fragments de tectable on a Southern blot, in contrast to the expectations based on the sequence of the cDNA clone. A preliminary analysis of a genomic cl one with most of the IP gene has indicated the presence of several int rons containing restriction sites detected by the Southern analysis. T his genomic clone was also used for subregional mapping by fluorescenc e in situ hybridization (FISH) to human metaphase chromosomes. A singl e locus in the region 1p35-36.1 was identified.