Z. Vidnyanszky et al., DIAZEPAM-BINDING INHIBITOR FRAGMENT 33-50 (OCTADECANEUROPEPTIDE) IMMUNOREACTIVITY IN THE CEREBELLAR CORTEX IS RESTRICTED TO GLIAL-CELLS, Glia, 10(2), 1994, pp. 132-141
The distribution of octadecaneuropeptide (ODN)-like immunoreactivity (
LI) and its relationship to gamma-aminobutyric acid (GABA)-LI were inv
estigated in the cerebellar cortex of adult rats with electron microsc
opy. At the electron microscopic level, ODN-LI was found exclusively i
n glial cells. In addition to Bergmann glia and its processes, cerebel
lar astrocytes were also labelled, encapsulating unlabelled neuronal e
lements of the cerebellum. These ODN-LI glial processes were observed
in close apposition to synaptic junctions, but immunoreactivity could
not be found in the synaptic cleft or in association with neuronal mem
branes. Since GABA-LI is always associated with neuronal elements, the
colocalization of GABA- and ODN-LI could not be confirmed in the cere
bellar cortex. Our results do not support the assumption that ODN is a
neuron-specific processing product of diazepam binding inhibitor. (C)
1994 Wiley-Liss, Inc.