STUDIES ON RED-CELL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE - EVALUATION OFREFERENCE VALUES

Citation
Gt. Yuregir et al., STUDIES ON RED-CELL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE - EVALUATION OFREFERENCE VALUES, Annals of clinical biochemistry, 31, 1994, pp. 50-55
Citations number
20
Categorie Soggetti
Biology,"Chemistry Medicinal
ISSN journal
00045632
Volume
31
Year of publication
1994
Part
1
Pages
50 - 55
Database
ISI
SICI code
0004-5632(1994)31:<50:SORG-E>2.0.ZU;2-P
Abstract
The analytical, intra-individual, inter-individual variation and refer ence values were determined for red cell glucose-6-phosphate dehydroge nase (G6PD). Different procedures for the conditions for storage of re d blood cells and the preparation of haemolysates were investigated. A total of 2170 samples of blood were taken from apparently healthy per sons-1212 males and 958 females-from randomly selected villages and ci ty centres in the southern part of Turkey. Analytical variation, intra -individual variation and inter-individual variation were 8.67%, 32.8% and 31.8%, respectively. The mean (SD) for G6PD was 8.6 (3.3) IU/gHb. The index of individuality, 1.03, showed that the reference intervals could be used for diagnostic purposes. Whole blood or a red cell pell et could be stored in physiological saline for one week at 4 degrees C or -20 degrees with little loss of activity. Two of three different p rocedures for the preparation of haemolysate gave data that showed no statistical difference and were equally satisfactory.