Gt. Yuregir et al., STUDIES ON RED-CELL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE - EVALUATION OFREFERENCE VALUES, Annals of clinical biochemistry, 31, 1994, pp. 50-55
The analytical, intra-individual, inter-individual variation and refer
ence values were determined for red cell glucose-6-phosphate dehydroge
nase (G6PD). Different procedures for the conditions for storage of re
d blood cells and the preparation of haemolysates were investigated. A
total of 2170 samples of blood were taken from apparently healthy per
sons-1212 males and 958 females-from randomly selected villages and ci
ty centres in the southern part of Turkey. Analytical variation, intra
-individual variation and inter-individual variation were 8.67%, 32.8%
and 31.8%, respectively. The mean (SD) for G6PD was 8.6 (3.3) IU/gHb.
The index of individuality, 1.03, showed that the reference intervals
could be used for diagnostic purposes. Whole blood or a red cell pell
et could be stored in physiological saline for one week at 4 degrees C
or -20 degrees with little loss of activity. Two of three different p
rocedures for the preparation of haemolysate gave data that showed no
statistical difference and were equally satisfactory.