STIMULATION OF TRUNCATED GLUCAGON-LIKE PEPTIDE-1 RELEASE FROM THE ISOLATED-PERFUSED CANINE ILEUM BY GLUCOSE-ABSORPTION

To elucidate whether glucose absorption is necessary for oral glucose- induced truncated glucagon-like peptide-1 (tGLP-1) release, we examine d tGLP-1 release by several stimulants from isolated perfused canine i leum. Glucose and nonabsorbable sugars, sucrose and 2-deoxyglucose (2D G), were intraluminally administrated. Following gel filtration of the perfusate after glucose ingestion, the main component of GLP-1 in the eluate is tGLP-1. Both glucose and tGLP-1 in the effluents increased after the intraluminal administration of 5 % glucose, but not by that of sucrose or 2DG. Glucose absorption is more important than contact o f glucose with ileal epithelium to stimulate tGLP-1 release.