Sl. Abidi et Tl. Mounts, REVERSED-PHASE RETENTION BEHAVIOR OF FLUORESCENCE-LABELED PHOSPHOLIPIDS IN AMMONIUM ACETATE BUFFERS, Journal of liquid chromatography, 17(1), 1994, pp. 105-122
Subcomponents of fluorescent derivatives of phosphatidylethanolamine (
PE) and phosphatidylserine (PS) were resolved by reversed-phase high-p
erformance liquid chromatography (HPLC) with mobile phases containing
acetonitrile, methanol, water, and ammonium acetate. fluorescence labe
led phospholipids (PL) include N-(rhodamine B sulfonyl)-PE, N-(7-nitro
benz-2-oxa-1,3-diazol-4-yl)-PE, N-(5-fluoresceinthiocarbamoyl) (FL)-PE
, N-(1-pyrenesulfonyl) -PE, and N-(5-dimethylaminonaphthalene-1-sulfon
yl)-PE. Among the compounds studied, FL-PE exhibited the highest degre
e of selectivity for component resolution. The HPLC behavior of the fi
ve PE derivatives was examined under variable concentrations of ammoni
um acetate. Capacity factors of the PL subcomponents increased with in
creasing concentrations of the acetate buffers. Incorporation of triet
hylamine into the mobile phase alleviated peak