S. Horii, LIQUID-CHROMATOGRAPHIC DETERMINATION OF OXYTETRACYCLINE AND CHLORTETRACYCLINE RESIDUES IN ANIMAL-TISSUES, Journal of liquid chromatography, 17(1), 1994, pp. 213-221
A simple and sensitive method has been developed for the simultaneous
determination of oxytetracycline and chlortetracycline in animal tissu
es by liquid chromatography(LC). Tissue samples were extracted with ED
TA-McIlvaine buffer(ph4.0)/methanol(3/7). The extracts were purified w
ith Sep-pak C18 and Bond Elut SCX cartridges. Two tetracyclines were s
eparated with a synthetic polymer based-ODS column under basic conditi
on. They were determined by LC with programmable fluorometric detector
.