REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC AND DERIVATIVEUV SPECTROPHOTOMETRIC DETERMINATION OF FAMOTIDINE IN PHARMACEUTICAL PREPARATIONS

A method for the determination of famotidine in tablets and vials is d escribed. The procedure is based on the use of the reversed-phase high -performance liquid chromatography, and of the second-derivative ultra violet spectra, by utilizing the linear relationship between drug conc entration and derivative peak amplitude. The minimum concentration det ectable by derivative spectrophotometry was 0.5 mu g/ml, and by HPLC 0 .1 mu g/ml. The relative standard deviations observed were approx. 1.5 % for derivative spectrophotometry, and 1.2% for HPLC. The proposed me thods, which give thoroughly comparable data, are simple and rapid, an d allow one to obtain precise and accurate results.