AMINO-ACIDS IN PNEUMOLYSIN IMPORTANT FOR HEMOLYTIC-ACTIVITY IDENTIFIED BY RANDOM MUTAGENESIS

Authors
HILL J ANDREW PW MITCHELL TJ
Citation
J. Hill et al., AMINO-ACIDS IN PNEUMOLYSIN IMPORTANT FOR HEMOLYTIC-ACTIVITY IDENTIFIED BY RANDOM MUTAGENESIS, Infection and immunity, 62(2), 1994, pp. 757-758
Citations number
15
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
62
Issue
2
Year of publication
1994
Pages
757 - 758
Database
ISI
SICI code
0019-9567(1994)62:2<757:AIPIFH>2.0.ZU;2-4
Abstract
The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identi fied substitutions within the cysteine-containing region and also at r esidues toward the N terminus of the toxin. These are the first report ed changes within the N-terminal region of the toxin that affect cytol ytic activity.