CO2-LASER NERVE WELDING - OPTIMAL LASER PARAMETERS AND THE USE OF SOLDERS IN-VITRO

To improve the welding strength, an in vitro study was performed to in vestigate the bonding strength of CO2 laser nerve welding (LNW), with and without the use of human albumin solution, dried albumin solution, egg white, fibrinogen solution, fibrin glue, and red blood cells as a solder. Fifteen different combinations of laser power(50, 100, and 15 0 mW) and pulse duration (0.1 to 3 s) were used with a spot size of 32 0 mu m. The results have been compared to suture, fibrin glue, and las er-assisted nerve repair (LANR). The strongest welds (associated with whitening and caramelization of tissue) were produced at 100 mW with p ulses of 1.0 s and at 50 mW with pulses of 3 s. The use of a dried alb umin solution as a solder at 100 mW with pulses of 1 s increased the b onding strength 9-fold as compared to LNW (bonding strength 21.0 +/- 8 .6 g and 2.4 +/- 0.9 g, respectively). However, positioning the nerves between cottons soaked in saline for 20 minutes resulted in a decreas e of the bonding strength (9.8 +/- 4.5 g). The use of a 20% albumin so lution and egg white, both at 50 mW with pulses of 3 s, resulted in a bonding strength of, respectively, 5.7 +/- 2.1 g and 7.7 +/- 2.4 g. Ot her solders did not increase the bonding strength in comparison to LNW . The substantial increase in bonding strength for some solders sugges ts that it is worthwhile to investigate the dehiscence rate and nerve regeneration of solder enhanced LNW in an in vivo study. (C) Wiley-Lis s, Inc.