CINNAMYL ALCOHOL-DEHYDROGENASE - IDENTIFICATION OF NEW SITES OF PROMOTER ACTIVITY IN TRANSGENIC POPLAR

Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-beta-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular a natomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. Feuillet, V. Lauver geat, C. Deswarte, C. Pilate, A. Boudet and J. Grima-Pettenati [1995] Plant Mol Biol 27: 651-657), the latter technique also allowed the det ection of other sites of promoter activity not revealed by the first t echnique. In situ hybridization confirmed the expression pattern obtai ned with the second sample preparation technique.