Ed. Kopczynski et al., RECOGNITION OF CHIMERIC SMALL-SUBUNIT RIBOSOMAL DNAS COMPOSED OF GENES FROM UNCULTIVATED MICROORGANISMS, Applied and environmental microbiology, 60(2), 1994, pp. 746-748
When PCR nas used to recover small-subunit (SSU) rRNA genes from a hot
spring cyanobacterial mat community, chimeric SSU rRNA sequences whic
h exhibited little or no secondary structural abnormality were recover
ed. They were revealed as chimeras of SSU rRNA genes of uncultivated s
pecies through separate phylogenetic analysis of short sequence domain
s.